A Research Study On Nutrient Agar - 1678 Words

The first patient, 18 year old female presented with symptoms of high fever, vomiting, diarrhoea, myalgia, hypotension and signs of septic shock. In order to identify possible bacteria responsible for these symptoms, a vaginal swab was sampled, incubated on agar overnight and stored at 4C, after which they were examined throughout a series of microbiological tests. The initial culturing of the sample on nutrient agar indicated two organisms were present, and hence these were analysed individually. A Gram stain was applied to each bacterium and they were viewed individually under microscope at 1000x oil immersion magnification. This specified both Gram negative diplobacilli, which will be referred to as organism one, and Gram positive staphylococci, which will be referred to as organism two. Organism one demonstrated irregular, flat, wavy, medium sized and cream coloured colony morphology when viewed on agar plate, however limited conclusive data can be obtained from these observations alone. In order to provide further information for diagnosis, additional culturing was conducted on a range of selective and differential media types including Horse Blood, MacConkey, Mannitol Salt and Brilliant chromogenic UTI agar’s. This demonstrated the organism was non-haemolytic, lactose fermenting, as evident through a red colony formation on the MacConkey agar, and unable to survive in high salt concentrations, as indicated through the lack of growth on Mannitol Salt agar. TheShow MoreRelatedCandida Rugosa Experiment737 Words   |  3 PagesThe experimental design of this research can be divided into five main stages. The first stage is development of yeast cell colony on nutrient agar and inoculum preparation of Candida rugosa ATCC 14830. The microorganism used in this res earch is isolated from natural soil. Second stage is extraction of lipase enzyme from the growth medium that contain olive oil that act as a substrate. Third stage of this project is preparation of immobilized and free enzyme culture system. The immobilized enzymeRead MoreCharacteristics Of Serratia Marcescens : History1016 Words   |  5 Pagespathogen only in the mid-1960, and the reason of those diseases was unexplained until the mid-1970s (J Ania, 2015). Taxonomy †¢ For many years, Serratia genus contained only S. marcescens species, and only since 1972 DNA homologies and other biochemical studies that were based on comparison have identified other species within the genus (Deguzman, 2012). Table 1. Taxonomic Hierarchy of S. marcescens TSN 958620 (ITIS, 2016). Kingdom Bacteria Cavalier (Smith, 2002) Subkingdom Negibacteria Cavalier (SmithRead MoreUnderstanding And Preservation Of Individual And Community Health3280 Words   |  14 PagesOne may wonder why research is important or why it is important to know how the things around came to be, exist, or interact with one another, particularly with respect to the microbial world. Knowledge that research provides is critical to the understanding of the known world around us. Experiments are what have helped to provide answers to questions, thus fostering knowledge, which allows us to enjoy the lives we live today. Research via experimentation is a critical component as the informationRead MoreFactors That Affect Our Soil Affects Them And Their Movements1098 Words   |  5 Pagesyou will be able to see through a microscope. I conducted a research on C. elegans to test to see how every day fertilizer that we apply to our soil affects them and their movements. When you are putting fertilizer on your soil you are adding extra nutrients to the soi l to make your yard look nicer. Although, if you are not careful you may also be putting too much fertilizer on your soil which is raising the concentration of all the nutrients in the soil abnormally Therefore, the reason for my experimentRead MoreCode Green Fluorescent in Bacteria1169 Words   |  5 Pageswidely used in the biotechnology field, for examples: In medicine, gene therapy involves transforming a sick person’s cell with healthy copies of the defective gene that causes the disease. In research, bacteria are transformed with genes encoding human proteins for Bio-manufacturing production or for further study of these proteins. Today’s lab, we are transforming bacteria with a gene that code for green fluorescent protein (GFP). This gene originally came from the bioluminescent jellyfish AequoreaRead MoreWhat Does Zno Nanoparticles Exhibit High Toxicity Against Bacteria But Minimum Effect On Human Cells?1716 Words   |  7 Pagessick due to the infections caused by eating food which is contaminated [11,12]. Studies have shown that Salmonella enterica is one amongst the more prevalent bacterial pathogens that causes food borne infections [11]. It is estimated that the medical and productivity losses caused by this bacterium were of high order. [11]. The interaction between NPs and microorganisms and biomolecules is an expanding area of research, which still requires to be largely explored. To the best of our knowledge thereRead MoreAseptic Technique and Transfer of Microorganisms2389 Words   |  10 Pagesbillions of cells (Tortora, Funke, Case, 2010, p.157). Although microbes can be found everywhere around us, such as soil, water, food, sewage, body surfaces and also air, but to grow microbes is laboratory for research purpose, different microbes may have different growth requirement. A nutrient material prepared for the growth of microbes in a laboratory is known as the culture medium. Some bacteria can grow well on just about any culture medium while the other required special media, and still othersRead MoreBacterial Enumeration of Various Meat Products1161 Words   |  5 Pages The objective of this study was to record a quantitative approximation of how many bacteria are present within various samples of meat products. T he bacterial content of each meat sample is vital information in regards to improvements within the meat processing industry, and gives reason for changing or sustaining current feeding and processing conditions. Understanding which methods taken in processing meat that is sold to the public is a matter of public health, as obtaining and maintaining lowerRead MoreThe Management of Wound Infections440 Words   |  2 PagesAntibiotic susceptibility pattern of Bacterial Strains Isolated from surgical or Non surgical lesions The study aimed to screen the bacterial pathogens present in surgical or non surgical wounds pus and to determine their antibiotic sensitivity and resistance pattern against 12 frequently used commercial antibiotics as Amikacin (AK10) Staphylococcus aureus (64.29%), Bacitracin (B10) Escherichia coli(27.77%), Cefotaxime (CTX) S.aureus (33.33%), Chloromphenicol (C) Pseudomonas aeruginosa(36.36%)Read MoreWhat Fabric Does The Best Job On Inhibit The Growth Of Bacteria, And Thus Microbiology3017 Words   |  13 Pagesknowledge of bacteria, and thus microbiology is required in order to not only complete the experiment, but to actually understand why this may be important in a person s everyday life. It may not seem to be, and it isn t, the most monumental piece of research that could be conducted, but it is useful piece of information nonetheless. Diseases, while not as easily spread as they once were considering how cleanliness is continuously, and quite possibly, annoyingly, but not unjustly, stressed in society